Overview:

Amplicon sequencing is a targeted sequencing method that targets specific genomic regions or conserved regions within the organisms to characterize them and study genetic variations. Amplicon sequencing selectively amplifies the target of interest using polymerase chain reaction (PCR), and then sequences regions of interest on sequencing platform. Amplicon sequencing includes 3 types: 16S rRNA sequencing (for bacteria), 18S rRNA sequencing, and ITS (Internal Transcribed Spacer) sequencing (for fungi).

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Applications:

• Detect mutations associated with different traits and inherited diseases, identify rare genetic mutations and low-frequency mutations.

• Detect genetic mutations associated with the cancers.

• Used in human leukocyte antigen (HLA) typing to determine compatibility for organ transplants or to study immune responses.

• Identify and characterize pathogens in clinical samples, monitor pathogens in wastewater and tracking viral variants.

• Identify genetic variations in crops and detect plant pathogens to manage crop diseases in agriculture.

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WorkFlow: