Overview:

Small RNA sequencing (small RNA-Seq) is a high-throughput sequencing technique used to study small RNA molecules, which typically range from 20 to 300 nucleotides in length. Small RNAs include a wide variety of non-coding RNAs, such as miRNAs, piRNAs, siRNAs and others. These small RNAs play essential roles in regulating gene expression and cellular processes. Small RNA sequencing allows for the comprehensive identification and quantification of these small RNAs across different conditions or tissue types.

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Applications:

• Provide insight into how miRNAs regulate gene expression and influence cellular processes like differentiation, apoptosis, and proliferation.

• Identify dysregulated miRNAs in cancer and some neurological diseases development, which can serve as potential biomarkers for diagnosis or therapeutic targets. miRNAs are often involved in cancer initiation, progression, and metastasis. Small RNAs also play crucial roles in neurogenesis and the regulation of genes associated with neurological diseases like Alzheimer’s, Parkinson’s, and autism.

• Track changes in miRNA expression during development, which provides insights into the regulation of gene networks that control processes like cell fate determination, differentiation, and morphogenesis.

• Study the small RNA response to infection in viral research.

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