Overview:

Bulk RNA sequencing (Bulk RNA-Seq) is a technique to provide insights into gene expression patterns, allowing researchers to understand how genes are regulated and expressed across different conditions, tissues, or experimental conditions. Bulk RNA-seq involves sequencing two types of libraries: mRNA-only library and whole transcriptome library that includes all RNA species except for rRNA.

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Applications:

• Provide insight into how miRNAs regulate gene expression and influence cellular processes like differentiation, apoptosis, and proliferation.

• Identify dysregulated miRNAs in cancer and some neurological diseases development, which can serve as potential biomarkers for diagnosis or therapeutic targets. miRNAs are often involved in cancer initiation, progression, and metastasis. Small RNAs also play crucial roles in neurogenesis and the regulation of genes associated with neurological diseases like Alzheimer’s, Parkinson’s, and autism.

• Track changes in miRNA expression during development, which provides insights into the regulation of gene networks that control processes like cell fate determination, differentiation, and morphogenesis.

• Study the small RNA response to infection in viral research.

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