Overview:

Methylation sequencing is a method of sequencing used to study DNA methylation patterns across the genome. DNA methylation is an important epigenetic modification that usually occurs at the Carbon-5 position of cytosine bases in cytosinephospho-guanine (CpG) dinucleotides. Methylation sequencing is based on detecting methylated cytosines (5-mC) in the DNA sequence. When DNA is treated with sodium bisulfite (the most common method), unmethylated cytosine is deaminated and converted to uracil which is read as thymine after PCR amplification prior to sequencing.

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Applications:

• Quantify DNA methylation and helps us better understand how DNA methylation influences cellular processes.

• Identify age-associated epigenetic markers to provide valuable information about the aging mechanism and age- related diseases like neurodegeneration.  

• Identify the diseases associated biomarkers to serve as the potential biomarkers for early diagnosis and prognosis.

• Evaluate the potential drugs impact on the DNA methylation patterns in drug discovery.

• Study how environmental factors modify the genome and contribute to diseases.

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WorkFlow: