Texas Scientific offers the single cell analysis utilizing the solutions of the 10x Genomics Chromium system. We offers the types of single cell sequencing below:

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• Single Cell Gene Expression (scRNA-seq) measures whole transcriptome gene expression in individual cells, offering a detailed view of cells functions and interaction within complex tissues. Tracing individual transcripts back to their cells of origin enables researchers to pinpoint unique gene expression profiles in highly heterogeneous samples, ultimately revealing rare cell types and dynamic cell states that other methods often miss.

• Single Cell Immune Profiling examines the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells on a cell-by-cell basis. With the addition of Feature Barcoding technology, it can be used to detect and analyze additional cellular readouts, such as cell surface markers and antigen specificity, to enhance immune cell phenotyping and study dynamic interactions between lymphocytes and target cells. The Chromium Single Cell Immune Profiling solution allows for simultaneous generation of full-length, paired V(D)J sequences, as well as gene expression, cell surface protein expression, and antigen specificity data at a single cell level.

• Single Cell Flex Gene Expression offers comprehensive scalable solution to measure gene and protein expression in samples that are fixed with formaldehyde to preserve fragile biology. Flex Gene Expression enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling.

 

Single Cell ATAC (Assay for Transposase-Accessible Chromatin): ATAC sequencing is an effective tool in the study of genome-wide chromatin accessibility landscapes and enables the identification of open chromatin regions, which are generally transcriptionally active genes. The sequencing of open chromatin regions can lead to the identification of transcription factors that are active in the phenotype or conditions being investigated and involve in important genetic pathways in the samples. However, many biological samples, such as tumors and tissues in different developmental states, contain multiple subpopulations of cells that potentially have different epigenomic profiles. Single cell ATAC-Seq is able to identify such cell subpopulations, and thus gives the most accurate description of chromatin state in these dynamic processes.

The Chromium Single Cell ATAC Solution adopted from 10X Genomics provides a comprehensive, scalable approach to determine the regulatory landscape of chromatin in hundreds to thousands of cells in a single sample. This is achieved by transposing nuclei in a bulk solution; then using a microfluidic chip, the nuclei are partitioned into nanoliter-scale Gel Beadsin-emulsion (GEMs). Sequencing-ready libraries are generated and sequenced, and the unique 10x barcodes are used to associate reads back to the individual partitions.